permeabilization buffer kit i (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems permeabilization buffer kit i
Permeabilization Buffer Kit I, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixation+permeabilization+buffer/Flow+Cytometry+Fixation+%26+Permeabilization+Buffer+Kit+I/pmc12901808-68-13-17
Average 94 stars, based on 65 article reviews

permeabilization buffer kit i - by Bioz Stars, 2026-10

94/100 stars

Images

Incubation:

Article Title: Generation of induced pluripotent stem cell line KUMi002-A with normal karyotype from a patient with Philadelphia chromosome-positive chronic myeloid leukemia.
Article Snippet: DAPI staining was performed and images were acquired using a fluorescence microscope (Olympus, Tokyo, Japan). .. Cells at passage 16 were detached using AccutaseTM (Stemcell Technologies, Vancouver, Canada) and incubated with fixation/permeabilization buffer (R&D Systems) for 30 min. iPSCs were stained with fluorescent-dye-conjugated antibodies, and isotype antibodies (Table 2) were used as negative controls. .. Flow cytometry was performed using FACS Canto (BD Bioscience, San Jose, CA, USA) and data were obtained using the FlowJo software.

Article Title: Generation of the human pluripotent stem cell lines KUMi005-A from a patients with multiple myeloma.
Article Snippet: The cells were incubated with secondary antibodies for 1 h. The nuclei were counterstained with 4′,6-diamidino2-phenylindole for 15 min before obtaining fluorescence images using a microscope (Olympus). .. iPSCs at passage 5 were dissociated with AccutaseTM (STEMCELL Technologies), and the detached cells were incubated with fixation/ permeabilization buffer (R&D) for 30 min at 4 ◦C. .. After washing with permeabilization/wash buffer (R&D Systems), the cells were incubated with each corresponding isotype control antibody (R&D Systems) for 30 min at room temperature (RT).

Article Title: Generation of a human induced pluripotent stem cell line KUMi006 from a patient with multiple myeloma.
Article Snippet: Images were acquired using a fluorescence microscope (Olympus). .. iPSCs detached using AccutaseTM(Stemcell Technologies) were incubated with fixation/permeabilization buffer (R&D Systems) and were then incubated with antibodies against SOX2 (1:20) and SSEA4 (1:20) for 30 min in the dark. .. A total of 20,000 iPSCs were prepared and analyzed by flow cytometry (FACSCanto II; BD Biosciences).

Article Title: Besides TLR2 and TLR4, NLRP3 is also involved in regulating Escherichia coli infection-induced inflammatory responses in mice.
Article Snippet: .. After washing, the cells were fixed and permeabilized with the Fixation/Permeabilization Buffer for 45 min, followed by resuspension with permeabilization buffer and incubation with an Alexa Fluor 488-conjugated anti-NLRP3 (5 μL/106 cells, 768319, R&D Systems, USA) antibody for 45 min at room temperature. .. The data were analyzed using FlowJo 10.0 software (TreeStar, USA).

Article Title: Generation of human induced iPSC KUMi003-A from acute promyelocytic leukemia (APL) M3.
Article Snippet: Images were captured using a fluorescence microscope (Olympus IX71). .. Cells at passage 7 were dissociated with AccutaseTM (Stemcell Technologies) and exposed to a fixation/permeabilization buffer (R&D) for 30 min. CLL iPSCs were incubated with fluorescent-conjugated antibodies for 30 min. .. The data were acquired using FlowJo software.

Article Title: Expression of CD200R1 and its Ligand CD200 on T-helper Lymphocytes of Pediatric Patients with Ulcerative Colitis and Crohn’s Disease
Article Snippet: Cells were incubated with FITC-labelled anti-CD4 (clone FAB3791F, R&D Systems) and APC-labelled anti-CD25 (clone BC96, R&D Systems) antibodies for 30 minutes at 4°C in the dark. .. For intracellular staining, after permeabilization with fixation/permeabilization buffer (R&D Systems), PE-labelled anti-Foxp3 antibody (clone IC8214P, R&D Systems) was added and incubated for 30 minutes at 4°C in the dark. ..

Article Title: A human pluripotent stem cell line KUMi004-A generated from a patient with chronic lymphocytic leukemia.
Article Snippet: Samples were PBS washed 3 times then secondary antibodies were incubated for 1 h. Following DAPI was stained before obtaining fluorescence images using a microscope (Olympus). .. iPSCs at passage 6 were detached with AccutaseTM (Stemcell Technologies) and incubated with fixation/ permeabilization buffer (R&D). .. These cells were reacted with fluorescent-labeled antibodies for 30 min at RT and detected using flow cytometry (BD).

Staining:

Article Title: Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.
Article Snippet: .. For IgA+ plasma cell analysis, LPMCs were stained with fluorescein isothiocyanate (FITC)–conjugated anti-rat IgA (MARA-1; AbD Serotec) and phycoerythrin (PE)-conjugated anti-rat CD45RA (OX-33; BD Pharmingen) antibodies after fixation and permeabilization using fixation/permeabilization buffer (R&D Systems) and PBS containing 0.1% saponin. .. For regulatory T-cell analysis, LPMCs were stained with FITC-conjugated anti-rat CD3 antibody (G4.18; BD Biosciences), PE-conjugated anti-rat CD4 antibody (OX-38; BD Biosciences), and Fixable Viability Stain 780 (BD Biosciences).

Review

permeabilization buffer kit i (R&D Systems)

Structured Review

Images

Related Articles

Incubation:

Staining:

Clinical Proteomics:

Cell Analysis:

Similar Products

Image Search Results